Ayurgenomic Profiling: Investigating Epigenetic Variations and Metabolic Responses Driven by Ayurvedic Dietary Protocols

DOI 10.66715/jsccr/2026.v3.i3.6374 | Original Research | 2026 | Volume 3 | Issue 3 | Page 90-98

1. Dr Ritesh Kumar, MD Scholar, All India Institute of Ayurveda, New Delhi, Email ID: ritesh.rk36@gmail.com, ORCID ID: 0009-0009-3336-0460

2. Dr Astha Raj, MD Scholar, All India Institute of Ayurveda, New Delhi, Email ID: drastharaj0987@gmail.com, ORCID ID: 0009-0008-9040-6843

3. Dr. Manoj Kumar, Tutor, Department of Pyhsiology, JHMC, WB

Corresponding Author:

Dr. Manoj Kumar, Tutor,

Department of Pyhsiology,

JHMC, WB

Ayurgenomic Profiling: Investigating Epigenetic Variations and Metabolic Responses Driven .

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Abstract

Background: Ayurgenomics is an emerging interdisciplinary field integrating the principles of Ayurveda with modern genomics, epigenetics, and systems biology to understand individual variability in health, disease susceptibility, and therapeutic responses. Ayurvedic dietary protocols, based on the concept of Prakriti (constitutional phenotype), are believed to modulate metabolic homeostasis through personalized nutritional interventions. However, the molecular mechanisms underlying these effects, particularly epigenetic modifications associated with dietary practices, remain insufficiently explored.

Objective: This study aimed to investigate epigenetic variations and metabolic responses associated with personalized Ayurvedic dietary protocols and to evaluate their relationship with constitutional phenotypes in healthy adults.

Methods: A prospective observational study was conducted among 120 participants aged 20–55 years classified into Vata, Pitta, and Kapha Prakriti using standardized Ayurvedic assessment tools. Participants followed individualized Ayurvedic dietary protocols for 12 weeks. Peripheral blood samples were collected at baseline and post-intervention to assess genome-wide DNA methylation patterns, selected histone modifications, and microRNA expression. Metabolic parameters, including fasting blood glucose, lipid profile, insulin sensitivity, inflammatory biomarkers, oxidative stress markers, and gut microbiome diversity, were also evaluated. Statistical analyses compared pre- and post-intervention changes and correlations between epigenetic markers and metabolic outcomes.

Results: Personalized Ayurvedic dietary intervention resulted in significant epigenetic remodeling, characterized by differential DNA methylation of genes involved in inflammation, glucose metabolism, lipid regulation, and mitochondrial function (p < 0.05). Significant improvements were observed in insulin sensitivity, serum lipid profile, inflammatory cytokines, oxidative stress indices, and gut microbial diversity compared with baseline. Distinct epigenetic signatures and metabolic responses were identified among the three Prakriti groups, suggesting constitution-specific biological adaptations. Correlation analysis demonstrated significant associations between altered DNA methylation profiles and improvements in metabolic biomarkers